光敏色素B正调控水稻叶绿素合成和叶绿体的发育

doi:10.3969/j.issn.10017216.2012.06.001

中国水稻科学 ›› 2012, Vol. 26 ›› Issue (6): 637-642.DOI: 10.3969/j.issn.10017216.2012.06.001

• 研究报告 • 下一篇

光敏色素B正调控水稻叶绿素合成和叶绿体的发育

赵杰^{1, 2} ,周晋军²,顾建伟²,钱凤芹²,谢先芝^{1, 2,*}

¹山东师范大学生命科学学院，山东济南 250014；²山东省农业科学院高新技术研究中心，山东济南 250100;

收稿日期:2012-01-16 修回日期:2012-04-15 出版日期:2012-11-10 发布日期:2012-11-10
通讯作者: 谢先芝1, 2,*
基金资助:
国家自然科学基金资助项目（30870192, 30971744）；转基因生物新品种培育科技重大专项（2009ZX08001029B）。

Phytochrome B Positively Regulates Chlorophyll Biosynthesis and Chloroplast Development in Rice

ZHAO Jie ^1,2, ZHOU Jinjun ², GU Jianwei ², QIAN Fengqin ², XIE Xianzhi ^1,2,*

¹ College of Life Sciences, Shandong Normal University, Jinan 250014, China; ² HighTech Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China;

Received:2012-01-16 Revised:2012-04-15 Online:2012-11-10 Published:2012-11-10
Contact: XIE Xianzhi1,2,*

摘要/Abstract

摘要： 水稻光敏色素基因家族包括3个成员，PHYA、PHYB 和PHYC，它们在调控水稻去黄化、花期和育性等光形态建成中具有重要作用。我们比较了白光和红光条件下野生型、phyA、phyB和phyAphyB突变体中叶绿素含量。结果表明，phyB感受红光正调控水稻叶绿素合成，phyA的作用仅仅在phyB功能缺陷时才能表现出来。叶绿素合成相关基因表达模式分析结果表明，水稻光敏色素介导的光信号主要通过调控原叶绿素酸酯氧化还原酶基因 (protochlorophyll oxidoreductase A，PORA) 的表达而影响叶绿素的合成。同时,还分析了phyB介导的红光信号对叶绿体发育的影响，结果表明，phyB介导的红光信号在调控叶绿体数目、基粒数目及叶绿体膜发育中具有重要作用。

关键词: 水稻, 光敏色素, 叶绿素合成, PORA基因, 叶绿体发育

Abstract: Phytochrome family in rice is composed of three members, PHYA, PHYB, and PHYC. Through characterizing the phytochrome mutants and wild type (WT) in terms of photomorphogenesis, roles of individual phytochromes have been preliminarily explored in regulating rice deetiolation, flowering time and fertility. We compared the chlorophyll contents of wild type and phyA, phyB and phyAphyB mutants grown either in white light (W) or in red light (R). The results suggest that phyB perceives red light to positively regulate chlorophyll biosynthesis, while the role of phyA can be detected only in the phyBdeficient mutant. Analyses on the expression levels of genes involved in chlorophyll biosynthesis revealed that phytochromes affected chlorophyll biosynthesis by regulating protochlorophyll oxidoreductase A(PORA ) expression. The role of phyB in chloroplast development was analyzed. The results suggest that phyB perceive red light to regulate chloroplast development by affecting the number of chloroplasts and grana, as well as the chloroplast membrane system.

Key words: rice, phytochrome, chlorophyll biosynthesis, PORA, chloroplast development

中图分类号:

赵杰1, 2 ,周晋军2,顾建伟2,钱凤芹2,谢先芝1, 2,*. 光敏色素B正调控水稻叶绿素合成和叶绿体的发育[J]. 中国水稻科学, 2012, 26(6): 637-642.

ZHAO Jie1,2, ZHOU Jinjun2, GU Jianwei2, QIAN Fengqin2, XIE Xianzhi1,2,*. Phytochrome B Positively Regulates Chlorophyll Biosynthesis and Chloroplast Development in Rice[J]. Chinese Journal of Rice Science, 2012, 26(6): 637-642.

参考文献

\[1\]Suetsugu N, Wada M. Chloroplast photorelocation movement mediated by phototropin family proteins in green plants. Biol Chem, 2007, 388: 927935.

\[2\]Franklin K A, Quail P H. Phytochrome functions in Arabidopsis development. J Exp Bot, 2010, 61: 1124.

\[3\]Bae G, Choi G. Decoding of light signals by plant phytochromes and their interacting proteins. Annu Rev Plant Biol, 2008, 59: 281311.

\[4\]Gu J, Liu J, Xue Y, et al. Phytochrome functions in rice development. Rice Sci, 2011, 18(3): 231237.

\[5\]Nagatani A. Lightregulated nuclear localization of phytochromes. Curr Opin Plant Biol, 2004, 7: 14.

\[6\]周波, 李玉花. 植物的光敏色素与光信号转导. 植物生理学通讯, 2006, 42(1): 134140.

\[7\]王静, 王艇. 高等植物光敏色素的分子结构、生理功能和进化特征. 植物学通报, 2007, 24(5): 649658.

\[8\]Takano M, Inagaki N, Xie X Z, et al. Distinct and cooperative functions of phytochromes A, B and C in the control of deetiolation and flowering in rice. Plant Cell, 2005, 17: 33113325.

\[9\]刘恒蔚, 周瑞阳, 徐俐. 光敏色素与光敏感雌性不育苎麻育性转换的关系.作物学报， 2006, 32(4): 597600.

\[10\]Chen M, Chory J. Phytochrome signaling mechanisms and the control of plant development. Trends Cell Biol, 2011, 21 (11): 664671.

\[11\]Takano M, Inagaki N, Xie X, et al. Phytochromes are the sole photoreceptors for perceiving red/farred light in rice. Proc Natl Acad Sci USA, 2009, 106: 1470514710.

\[12\]Reed J W, Nagpal P, Poole D S, et al. Mutations in the gene for red/farred light receptor phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development. Plant Cell, 1993， 5: 147157.

\[13\]Reed J W, Nagatani K, Elich T D, et al. Phytochrome A and phytochrome B have overlapping but distinct functions in Arabidopsis development. Plant Physiol, 1994, 104: 11391149.

\[14\]Casal J J, and Mazella M A. Conditional synergism between cryptochrome 1 and phytochrome B is shown by the analysis of phyA, phyB, and hy4 simple, double, and triple mutants in Arabidopsis. Plant Physiol, 1998, 118(1): 1925.

\[15\]McCormac A C, Terry M J. Loss of nuclear gene expression during the phytochrome Amediated farred block of greening response. Plant Physiol, 2002, 130: 402414.

\[16\]史典义，刘忠香，金危危. 植物叶绿素合成、分解代谢及信号调控. 遗传, 2009, 31(7): 698704.

\[17\]吴自明, 张欣，万建民. 叶绿素生物合成的分子调控.植物生理学通讯, 2008, 44(6): 10641070.

\[18\]Wu Z, Zhang X, He B, et al. A chlorophylldeficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol, 2007, 145(1): 2940.

\[19\]Moon J, Zhu L, Shen H, et al. PIF1 directly and indirectly regulates chlorophyll biosynthesis to optimize the greening process in Arabidopsis. Proc Natl Acad Sci USA, 2008, 105: 94339438.

\[20\]Stephenson P G, Christian F, Matthew J T. PIF3 is repressor of chloroplast development. Proc Natl Acad Sci USA, 2009, 106: 76547659.

\[21\]Dehesh K, Tepperman J, Christensen A H, et al. phyB is evolutionarily conserved and constitutively expressed in rice seedling shoots. Mol Gen Genet, 1991, 225: 305313.

\[22\]Kay S A, Keith B, Shinozaki K et al. The sequence of the rice phytochrome gene. Nucleic Acids Res, 1989, 17: 28652866.

\[23\]Basu D, Dehesh K, SchneiderPoetsch H J, et al. Rice PHYC gene: Structure, expression, map position and evolution. Plant Mol Biol, 2000, 44: 2742.

\[24\]Xie X, Shinomura T, Inagaki N, et al. Phytochromemediated inhibition of coleoptile growth in rice: Agedependency and action spectra. Photochem Photobiol, 2007, 83: 131138.

\[25\]刘婧, 柳艳梅, Takano M, 等. 光敏色素影响赤霉素调控的水稻幼苗光形态建成特征. 科学通报, 2010, 55: 23842390.

\[26\]Xie X, Xue Y, Zhou J, et al. Phytochromes regulate SA and JA signaling pathways in rice and are required for developmentally controlled resistance to Magnaporthe grisea. Mol Plant, 2011, 4(4): 688696.

\[27\]Takano M, Kanegae H, Shinomura T, et al. Isolation and characterization of rice phytochrome A mutants. Plant Cell, 2001, 13: 521534.

\[28\]张其德. 测定叶绿素的几种方法. 植物学通报, 1985, 3: 6064.

\[29\]Neff M M, Chory J. Genetic interactions between phytochrome A, phytochrome B, and cryptochrome 1 during Arabidopsis development. Plant Physiol, 1998, 118(1): 2735.

\[30\]Batsuschauer A, Apel K. An inverse control by phytochrome of the expression of two nuclear genes in barley (Hordeum vulgare L.). Eur J Biochem, 1984, 143(3): 593597.

\[31\]Msinger E, Batschauer A, Schfer E, et al. Phytochrome control of in vitro transcription of specific genes in isolated nuclei from barley (Hordeum vulgare)．Eur J Biochem, 1985, 147: 137142.

\[32\]Huq E, AlSady B, Hudson M, et al. Phytochromeinteracting factor 1 is a critical bHLH regulator of chlorophyll biosynthesis. Science, 2004, 305: 19371941.

\[33\]Rüdiger W, Benz J, Guthoff C. Detection and partial characterization of activity of chlorophyll synthetase in etioplast membranes. Eur J Biochem,1980, 109: 193200.

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光敏色素B正调控水稻叶绿素合成和叶绿体的发育

Phytochrome B Positively Regulates Chlorophyll Biosynthesis and Chloroplast Development in Rice

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